Interconversion of rec+ and rec- Phenotype in Lysogens of Escherichia coli
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چکیده
منابع مشابه
Expression of cloned bacteriophage T4 uvsW and uvsY genes in rec+ and rec- Escherichia coli.
Chimeric plasmids containing the uvsY uvsW region of the T4 genome were examined for the expression of these genes. Certain of these plasmids were shown to express the uvsY or the uvsW gene products by their ability to complement the UV sensitivity of infecting uvsW or uvsY mutant phage. Also, a chimeric plasmid containing both the uvsW and uvsY genes increases the survival of UV-irradiated, me...
متن کاملBiochemical and genetic studies of recombination proficiency in Escherichia coli. II. Rec+ revertants caused by indirect suppression of rec- mutations.
All recB(-) and recC(-) mutants of E. coli carry out significant residual genetic recombination, whereas all recA(-) mutants form no recombinants. This observation suggests that an alternative minor pathway of recombination, independent of recB(+) and recC(+) products, may be operative in Escherichia coli. Rec(+) revertants of recB(-)recC(+), recB(+)recC(-), and recB(-) strains of E. coli have ...
متن کاملRole of DNA polymerase I and the rec system in excision-repair in Escherichia coli.
The DNA polymerase I-deficient mutant polA1 is shown to perform an increased amount of UV-stimulated repair synthesis relative to its pol(+) parent. In contrast, a recA recB double mutant is found to perform little detectable repair synthesis. Analysis of the density distribution of sheared DNA of the recA recB mutant reveals that none of the repair synthesis in this strain is in the large repa...
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Both the RecBCD pathway, known for its involvement in homologous recombination and repair of double stranded DNA breaks, and the RecFOR pathway may recognize the linear fragments of DNA that are introduced to the cell during transposition, affecting the transposition frequency. The exact nature of how Rec pathways are involved in transposition is a current topic of research. In this study, usin...
متن کاملPhage X Has an Analog of Escherichia coli rec 0 , recR and recF Genes
The RecF pathway catalyzes generalized recombination in Escherichia coli that is mutant for recBC, sbcB and sbcC. This pathway operating on conjugational recombination requires the recA, recF, recJ, recN, rec0, recQ, recR, ruvA, ruvB and ruvC genes. In contrast, X mutant for its own recombination genes, int, reda and red& requires only the recA and reg genes to recombine efficiently in recBC sb...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1969
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-5-2-171